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KMID : 1035420190070030085
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Journal of The Korean Society of Integrative Medicine
2019 Volume.7 No. 3 p.85 ~ p.94
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A Study of the Mask and Hand Contamination in Dental Clinic
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Pyo Eun-Ji
Lee Kyung-Hee
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Abstract
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Purpose: The purpose of this study was to observe the degree of mask contamination in dental hygienist for general and oral bacteria and to identify areas of mask contamination after treatment.
Methods: Masks were collected with every fifty dental hygienists who currently working in the department of preventive dentistry, prosthodontics, and orthodontics in Busan. The mask bacteria were collected in specific upper and side parts of the mask. Hand germs were collected using sterile cotton swabs, and then placed in a sterile conical tube. These were transferred to the laboratory. Hand germs and mask bacteria were incubated with nutrient broth (NB) and brain heart infusion broth (BHI) for 24 hrs and each cultured with NB and BHI plate at 37 ℃ for 48 hrs. Collected data were analyzed using the SPSS Window 20.
Results: The number of bacteria was observed in the order of the department of preventive dentistry (10.1×105CFU/ml), prosthodontics (14.7×105CFU/ml), and orthodontics (23.3×105CFU/ml) in the hand. In general bacteria, the difference of contamination was seen by the parts of the mask, but there was no significant difference. However, the oral bacteria were observed highly contaminated upper part of the mask in preventive dentistry. The mask contamination according to the medical departments was observed. Especially, the contamination of mask in preventive dentistry was significantly higher than other departments in oral bacteria.
Conclusion: This study suggested that correct mask replacement and recognition of contamination areas can contribute to the prevention of infectious disease. and it would be necessary to increase hand hygiene performance to prevent cross-infection with masks. Also, this study may give an idea for making guidelines for mask management and supporting to establish clear criteria for the education program of personal protective equipment.
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KEYWORD
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Infection, Masks, Oral bacteria, Protective equipment, Spatter
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